Recombinant adenovirus vectors are the preferred method for a wide range of in vitro and in vivo gene delivery applications. Convenient purification of smaller quantities of these vectors at a high level of purity and consistency has remained a challenge. Cesium chloride gradients are laborious and take two days. Alternatives based on chromatography have been inconsistent and cumbersome.
The Adenopure® Kit may be used to purify recombinant adenovirus vectors without the need for time-consuming and labor-intensive 2X CsCl gradients. A novel disposable Adenopump® syringe/pump and membrane design achieves the highest quality adenovirus preparations and substantially improved yields in a 90-minute procedure.
The Adenopure Adenovirus Purification Kit offers several important advantages:
- Less Starting Material - Higher
Yield: Obtain up to 5x1012 viral particles
from as few as five 150cm2 plates of
starting material. This is more than twice the yield
from a typical 2X CsCl prep or from other kits.
- Higher Quality: Adenopure produces
fewer inactive viral particles (15:1 VP:IU ratio) and
less residual endotoxin (<3EU/ml) than other
methods. The product is suitable for all routine
applications including in vitro infections, tranductions
and animal studies
- Easy and Fast: This simple, convenient procedure can be completed in 90 minutes after harvest of the cell pellet or cell supernatant.
The Adenopure® Single Use Kit Kit contains all the components
required to perform one (1) virus isolation, including
Benzonase® and syringes. |
The Adenopure® Kit Kit contains all the components
required to perform three (3) virus isolations, including
Benzonase® and syringes. |
The kit may be purchased by calling (888) 245-9182 or (610) 640-0800 or by email at Adenopure@puresyn.com.