Adenoviral vectors have become a preferred method for a wide range of in vitro and in vivo gene delivery applications, but convenient large-scale purification of adenovirus has remained a challenge.
Finally, a pure, simple and effective alternative to 2X CsCl gradient ultracentrifugation is available in a convenient 125-minute kit that yields up to 1x1014 virus particles from as few as fifty plates of starting material. Adenopure LS® (large-scale) is optimized to produce exceptionally pure adenovirus preparations with a simple procedure.
The Adenopure LS Adenovirus Purification Kit offers several important advantages:
- Less Starting Material - Higher
Yield: Obtain up to 1x1014 viral particles
from fifty 150cm2 plates of starting
material. This is more than twice the yield from
a typical 2X CsCl prep or from other kits.
- Higher Quality: Adenopure LS produces
fewer inactive viral particles (20:1 VP:IU ratio) and
less residual endotoxin (<3EU/ml) than other
methods. The product is suitable for all routine
applications including in vitro infections, tranductions
and animal studies.
- Easy and Fast: This simple, convenient procedure can be completed in 2 hours after harvest of the cell pellet or cell supernatant.
The Adenopure LS Adenovirus Purification Kit (Catalogue No. 70500) sells for $795.00 plus shipping and handling. Each kit contains all the components required to perform one (1) virus purification, including Benzonase® and syringes.
The kit may be purchased by calling (888) 245-9182 or (610) 640-0800 or by email at Adenopure@puresyn.com.