Adenoviral vectors have become a preferred method for a wide range of in vitro and in vivo gene delivery applications, but convenient large-scale purification of adenovirus has remained a challenge.

Finally, a pure, simple and effective alternative to 2X CsCl gradient ultracentrifugation is available in a convenient 125-minute kit that yields up to 1x10¹⁴ virus particles from as few as fifty plates of starting material. Adenopure LS^® (large-scale) is optimized to produce exceptionally pure adenovirus preparations with a simple procedure.

The Adenopure LS Adenovirus Purification Kit offers several important advantages:

• Less Starting Material - Higher Yield: Obtain up to 1x10¹⁴ viral particles from fifty 150cm² plates of starting material. This is more than twice the yield from a typical 2X CsCl prep or from other kits.
  
• Higher Quality: Adenopure LS produces fewer inactive viral particles (20:1 VP:IU ratio) and less residual endotoxin (<3EU/ml) than other methods. The product is suitable for all routine applications including in vitro infections, tranductions and animal studies.
  
• Easy and Fast: This simple, convenient procedure can be completed in 2 hours after harvest of the cell pellet or cell supernatant.

The Adenopure LS Adenovirus Purification Kit (Catalogue No. 70500) sells for $795.00 plus shipping and handling. Each kit contains all the components required to perform one (1) virus purification, including Benzonase^® and syringes.

The kit may be purchased by calling (888) 245-9182 or (610) 640-0800 or by email at Adenopure@puresyn.com.