The Adenopure LS ® Adenovirus Purification
Kit couples membrane adsorber technology with proprietary
buffer formulations to provide rapid and simple isolation
of highly purified adenovirus preparations suitable
for in vitro biological assays.
The Adenopure LS kit contains is capable of purifying up to 1x1014 viral particles, yielding titers
of up to 5x1013 viral particles. The
Adenopure LS Kit includes Benzonase® endonuclease
for reduction of contaminating nucleic acids in crude
Adenovirus lysates prior to purification.
Adenopure LS relies on a disposable membrane cartridge
to generate high quality adenovirus preparations
without ultracentrifugation or technically demanding
and time consuming steps.
Adenopure LS works well with two- to five-day
infected cell suspensions and generates high-purity
adenovirus that rivals traditional 2X CsCl purification.
The potency of the adenovirus preparation is what
really counts! Adenopure LS purification results
in virus particle to infectious unit ratios that
are typically in the 20:1 range, at least two to
three times better than 2X CsCl.
Adenopure LS preparations are low in contaminating
endotoxin (<3EU/ml), a particularly important consideration
for in vivo applications.
*See our poster presented at the Seventh
annual ASGT meeting on our web site.
For more information please contact (610) 640-0800 or email cs@puresyn.com