The Puresyn Plasmid purification process uses our proprietary resin, PolyFlo®, to purify supercoiled plasmid from crude lysate prepared using standard alkaline lysis methods in the absence of RNAse treatment or detergent extraction. PolyFlo® is a chemically inert non-porous polymer. The PolyFlo® purification process uses principles of ion-paired reverse-phase chromatography for separation of nucleic acid species based on the combination of differences in hydrophobicity and charge density. The addition of the ion-pairing agent TBAP to a crude lysate creates the ion-pair bridge between the phosphate backbone of DNA and the hydrophobic PolyFlo® matrix. The strength of the binding is based on the number of phosphates (size) and charge density. This permits simultaneous separation of RNA, chromosomal DNA from plasmid as well as non-supercoiled isoforms, such as open circular plasmid from supercoiled plasmid. The inherent hydrophobic nature of the resin allows for removal of contaminating endotoxins during the process as well.

A Drug Master File for PolyFlo® is on file with the US FDA.